In 1994, Dry and Robinson described the molecular cloning and characterization of grape berry PPO. Southern analysis suggested the presence of only one gene in the grapevine.
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To further study the BY55 molecular structure, we cloned the gene encoding BY55 by COS cell expression cloning. The cDNA sequence predicts a cysteine-rich, glycosylphosphatidylinositol (GPI)-anchored protein of 181 amino acids with a single… A real-time polymerase chain reaction (real-time PCR), also known as quantitative polymerase chain reaction (qPCR), is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). Molecular cloning is the laboratory process used to create recombinant DNA. It is one of two most widely used methods, along with polymerase chain reaction (PCR), used to direct the replication of any specific DNA sequence chosen by the… is one of the most commonly used procedures in molecular biology. A gene of interest may be inserted into a plasmid vector via ligation, and the plasmid is then transformed into Escherichia coli cells. Molecular Cloning: A Laboratory Manual 3rd Ed. Cold Spring Harbor Laboratory Press. Cold Spring Harbor, NY. Analysis of protease-digested glycoprotein by reversed-phase HPLC. Peptides were obtained after digestion of reduced and alkylated glycoprotein with trypsin or protease V8. A, HPLC profile of trypsin-digested glycoprotein. Final Report of Molecular Biology - Free download as PDF File (.pdf), Text File (.txt) or read online for free.
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